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ANTIBACTERIAL ACTIVITY PEGANUM HARMALA PDF

Download Citation on ResearchGate | Antibacterial activity of Peganum harmala | Successive petroleum ether, chloroform and methanol extracts of Peganum. Successive petroleum ether, chloroform and methanol extracts of Peganum harmala seeds were tested (in vitro) for their antibacterial activity. examine the antibacterial activities of Peganum harmala extracts on the Key words: Antimicrobial effect, Biofilm, Peganum harmala, Pathogenic bacteria.

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Zygophyllaceae is one of the most famous medicinal plants used in traditional medicine of Iran. The aim of this study was to consider antibacterial effects of the methanolic extract of different parts of P. Antibacterial properties of methanolic extract of mentioned parts were assessed by disc diffusion antibwcterial.

Active extract was fractioned using Thin Layer Chromatography; also their synergism activity in combination with synthetic antibiotic was evaluated. Among the evaluated parts of P.

Antibacterial effect of leaf part was moderate while stem and flower extracts showed relatively poor activity. Antibacterial activity of root extract against most antibacterlal the tested Gram positive bacteria was better hxrmala seed extract. Tested against Gram negative bacteria the obtained results were inconsistent. TLC Thin Layer Chromatography results revealed that seed and root extracts were different in terms of nature and content of their constituents.

Furthermore, these two extracts showed an excellent stability to temperature and pH treatment. Also, the seed and root extracts showed synergism in combination with zctivity, colistin and carbenicillin. Nowadays, bacterial infections especially those caused by multi-drug resistant MDR bacteria have become one of the great challenges for modern healthcare. Therefore, discovering new antibacterial compounds with improved activity is necessary.

Since the pehanum time, the idea of finding healing powers in plants has been noteworthy. Plants produce a vast array of secondary metabolites that in many cases, these substances act as defense mechanisms against predation by herbivores, microorganisms and insects; also similar substances can be produced by plants as a part of their normal growth and development or in response to stress Mirjana et al.

Zygophyllaceaethat is also called Harmal, Suryin Rue, is a perennial, bushy, and activiyy flowering plant with short creeping root which may grow to harmxla high Shamsa et al. It’s native to semi-arid rangeland, steppe area and sandy soils Mahmoodian et al. This plant has been considered for the treatment of a variety of human ailments, such as lumbago, asthma, colic, jaundice and as a stimulant emmenagogue Bukhari et al.

The most pharmacological active compounds of P. There are various reports that P. Also, it has been reported that this plant had antibacterial, antifungal and antiviral effects Shonoudam et al. However, a study with focusing on antibacterial activity of different parts of P. The objective of the present study was to assess the antibacterial property of the methanol extract from different parts of P.

Different parts of P. Sample collection was done in April for leave and stem, in May for flower, in June for seed and in September for root. The leaf, flower, seed and stem were shade dried at room temperature for ten days while the root was shade dried for twenty days. These mentioned parts were ground to a fine powder. The powder 1 g was extracted using 10 ml of methanol-distilled water solution 8: This process was repeated for three times. Then, solvent removed by evaporation Darabpour et al.

A total of thirteen bacteria were selected for this study. Gram positive bacterial species were Bacillus anthracisBacillus cereusBacillus pumilusStaphylococcus aureusStaphylococcus epidermidisListeria monocytogenes and Streptococcus pyogenes.

Gram negative bacterial species were Pseudomonas aeruginosa, Brucella melitensisProteus mirabilisSalmonella typhiEscherichia coli and Klebsiella pneumoniae.

These species were originally isolated from clinical specimens and identified by standard biochemical reactions based on morphological and biochemical characters according to the methods described in Bergey’s manual for systematic bacteriology Garrity et al. Different characteristics were Gram stain, pigmentation, motility and utilization of different carbon and nitrogen sources and so on.

The turbidimetry of the suspension was adjusted to the McFarland 0. At first, a total of 0. The lawn culture was prepared by sterile cotton swab and allowed to antibacteriql in contact for 1 min. The sterile filter paper discs 6 mm diameter Cermelli et al. This experiment was carried out in duplicate. All of these synthetic antibiotic discs were prepared from Difco. In the tube dilution assay, standard bacterial suspension and different concentrations of extract 0.

The first tube in the above series without sign of visible growth was considered as the MIC. The least concentration that inhibited colony formation on agar was considered as MBC for the extract.

To determine the synergistic effect of the most effective parts of P. Finally, the result of these treatments was found by measurement of the inhibition zone against one of the most important human pathogenic bacterium. The methanolic extracts of the most effective parts of P. The separated components were visualized under visible and ultraviolet light and nm. Also, the retardation factor R f values of the spots were determined.

On the basis of the obtained results that are shown in Figures Fig. Increasing the antibacterial activity of the different parts of P.

Among Gram positive bacteria, S. Antibacterial activity of the root extract against tested Bacillus species and S. Moreover, the seed extract of P. The flower, leaf and stem extracts of P. Among the used parts of P.

All of the tested clinical bacteria were MDR strains. Also, all of the tested bacteria were resistant to colistin.

Antimicrobial Activity of Peganum Harmala L. on Streptococcus mutans Compared to % Chlorhexidine

Considering antibacterial activity of the different parts of P. Also, these values for seed extract against E. Moreover, MIC values for seed and root extracts antibadterial B. Based on the results from pH treatment Figure 7 Fig. The observed zone of inhibition after treatment of the seed extract with the different temperature was equal peganu, untreated extract 20 mm Figure 8 Fig.

In general, antibacterial activity of these two extracts was not considerably affected after temperature and pH treatment.

As we can see in Figures 9A and B Fig. Some spots are more intensive in one extract comparing with other extract. In general, the obtained results from TLC revealed that these two parts of P. The results of the combination of seed and root extracts of P. Combination of seed and root extract with novobiocin against E.

Synergistic effect of seed extract in combination with novobiocin against E. Furthermore, the observed synergism of root extract with colistin against E. Combination of the root extract of P. Meanwhile, the seed and root extract in combination with carbenicillin showed a relatively weak synergistic activity against MRSA. In recent years, an explosive spread of MDR bacterial pathogens has become a serious concern worldwide in terms of public health and economic effects. Medicinal plants have been shown to be a potential source of pharmaceutical natural products.

The comparison between pharmaceutical effect of different parts of a medicinal plant can give a good vision for accomplishing further study with more efficiency.

In this study, for the first time we have attempted to compare antibacterial activity of the different parts of P. Each of the used parts of P. Among the evaluated different parts of P. The seed and root are known as reservoir parts in plants, so, it is very likely that these parts served as arsenal of the secondary metabolites with antibacterial activity in P.

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harmapa Based on the obtained results, the root extract has a better antibacterial activity against Gram positive bacteria except L. Thin Layer Chromatography can justify this difference, because harmaoa TLC results for seed and root extract showed that these extracts contain several bands with different density or even some bands exist in one extract that we have not been able to find in another one. So, the content of each of the constituents and nature of constituents for these two extracts can be the determinant factors in their antibacterial mechanisms.

Unlike other Gram positive bacteria, L.

K 1 which has been reported antibacterizl Arshad et al. Antibacterial effect of antibxcterial extracts against K. For instance, pH stability is important in the treatment of brucellosis, one of the most important factors for the treatment of brucellosis is that the antibiotic should be able to act under acidic condition pH 5 anfibacterial phagolysosomes of macrophages because brucellae grow and replicate in this pH value Akova et al. Thus, considering antibacterial activity of the seed and root extracts of P.

Nowadays, development of bactericidal agents against MRSA as the most important causative agent of nosocomial infections is valuable so antibacterial activity of seed and root extract of P.

Our results indicate that crude extract of P. In general, combination therapy such as one resulted from synergism between antibiotic and plant extract can be used to as new choice for treatment of infections caused by MDR pathogens, to expand antibacterial spectrum, to prevent emergence of MDR pathogens and to minimize the possible toxic effects Aiyegoro et al.

Also, synergism between plant extract and synthetic antibiotics can develop standardization of herbal medicine for treatment and prevention of infectious diseases. Unlike the results that antibadterial shown in Figures 1 Fig. Colistin is an antibiotic that affects the cell membrane of bacteria. So, it is likely that the active phytochemicals, which exist in root extract, antibcterial with cell-wall petidoglycan synthesis in bacteria more than seed extract and finally resulted in increasing of the access of colistin to the cell membrane, the outcome of this event is actifity the synergistic effect.

On the other hand, the active efflux pump in E. So, may these extracts interfere with this pump and assumed as resistance modifying agents McKeegan et al. So far, several alkaloids with pharmaceutical activity including harmine, harmane, harmalol, harmaline, vasicine, vasicinon and peganine have been obtained from the various parts of this plant Fathiazada et al.

It has been reported that harmane as a highly aromatic planar alkaloid exerts its antibacterial activity through interchalate antibacteriwl DNA Cowan, [ 12 ]thus, this antibacterial mechanism must be considered for active extract of P.

CALDERAS DE VAPOR ACUOTUBULARES PDF

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Discussion in ‘ Specialized Terminology ‘ started by EmphyrioAug 29, Log in or Sign up.

Caldera acuotubular/acuotubulares | WordReference Forums

Dictionary and thread title search: Previous Thread Next Thread. Emphyrio New Member Mauritius French. Hello, I am trying to translate the following sentence but am a bit lost.

How would ve translate “calderas acuotubulares” into Spanish? Does my translation makes any sense to the engineers around? Could someone give me a hand?

EmphyrioAug 29, Hi Emphyriogood to see you again I suppose you meant ” into English “, right? Steam is produced in water-tube boilers, which are filled with water resulting from condensation of steam resulting from the [first evaporation??? I’m only going “slightly mental” over this translation I’m no translator, no engineer, and no remolacha lover anymore Thanks for the ” water-tube boiler “, that most helpful! As for the text, that’s what it is I guess that “evaporacion”, as you rightly pointed out, refers to condensate form another process God himself might not know I “dangerously assumed” that it was the one from the first evaporation I shall change that to “one of the acuotuulares processes”.

Firetube boiler

Do you, by the way, know what CV means not Curriculum V? Many thanks for your repeated help Thank you “un milion de veces”!

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INPO AP 928 PDF

Support for INPO AP Schedule Adherence. P6 Analytics provides out-of- the box support for the Institute of Nuclear Power Operations (INPO) Work. Support for INPO AP Schedule Adherence. INPO AP, Work Management Process Description. • INPO , Guideline for Excellence in Procedure and Work Instruction Use and Adherence. 7.

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Impo for sale nor for commercial use. All other rights reserved. Neither Ipo, INPO members, INPO participants, nor any person acting on the behalf of them a makes any warranty or representation, expressed or implied, with respect to the accuracy, completeness, or usefulness of the information contained in this document, or that the use of any information, apparatus, method, or process disclosed in this document may not infringe on xp owned rights, or b assumes any liabilities with respect to the use of, or for damages resulting from the use of any information, apparatus, method, or process disclosed in this document.

The process provides the fundamental structure for efficient use of station resources as well as overall improvement of equipment condition. This is an evolutionary document that incorporates current best industry practices and that will be revised periodically to document process improvements as they are developed. This revision provides clearer definitions of work classification along with discerning examples —see Appendix H as well as defining why they are important and how they are to be used.

It also provides updated guidance on industry initiatives to improve the work management process, such as work prioritization Appendix Drevised performance measures Appendix Egraded work package planning Appendix Fand graded work scheduling Appendix G.

The work management process is one of an integrated set of processes for the operation and support of nuclear plants. A number of standard definitions have been developed to facilitate comparison and benchmarking among plants to improve overall industry performance.

Likewise, a suggested set of core performance indicators is included to help measure and monitor work management trends and process effectiveness. The work management process description reflects the experience gained from work management assistance visits to operating plants and benchmark trips to domestic and international utilities. The description contains the elements considered essential to a wellfunctioning work management process, as well as options now being employed at a pa of plants as their processes have matured.

This process document revision was developed with the direct participation of a Work Management Working Group consisting of representatives from several utilities actively involved in improving and reengineering their own processes.

This information will be considered in a future revision to the document. Please direct comments to: This document will strive to establish understanding of the guiding principles for work management and how they are 298 and supported by the 298 defined within.

To ensure nuclear safety by providing timely identification, selection, planning, coordination, and execution of work necessary to maximize the availability and reliability of station equipment and systems 2. To manage the risk associated with conducting work 3. To identify the impact of work to the station and work groups and to protect the station from unanticipated transients due to the conduct of work 4.

Proper characterization and prioritization of work are basic to this first ino. Characterization of work into Corrective, Elective, or Other maintenance will help stations understand the overall health of their systems and components.

Not all corrective and elective maintenance is the same, so these classifications alone cannot sufficiently establish ino work should be accomplished first. Stations must not overly emphasize the significance of corrective maintenance, based solely on the classification of the a; alone.

True significance can only be ascertained when the work classification is used in conjunction with the system and operational significance of the equipment or system that the work is on.

The revised definitions for these three classifications of work, coupled with an effective prioritization process see Appendix Dwill define why and when work should be accomplished. The second principle deals with the management not the elimination of a. This impact could be as simple as unanticipated control room alarms, to actual equipment or system impact that requires the entry ao a limiting condition for operation LCOto plant transients and shutdown.

It could also a the impact on station work groups based on unanticipated support needs. The last principle recognizes that station resources of material and people are a limited commodity and, as such, must be fully used to maximize innpo value of and minimize the cost of maintaining station equipment.

The behaviors in this document embody the above principles. Appendix I provides specific details on each of these attributes and objectives. An effective work management process should do the following: Promote and improve nuclear, industrial, and radiological safety performance. Improve equipment performance and system health. Increase productivity and reduce costs through the efficient use of resources.

INPO AP 工作管理过程描述_图文_百度文库

A determination of productivity and manpower use should be examined. An effective FIN fix-it-now process and short-cycle process are part of this objective. Provide for a long-range plan to include major design changes and predictive and periodic maintenance activities. This should include provisions to address equipment obsolescence and asset management. Integrate all station organizations into the process, providing them with an understanding of the process, their contribution to the process, and the associated accountability and commitment to the process.

Integral to this culture is line ownership and accountability. Provide a proper methodology for work prioritization to ensure the right station work is done in the right time frame.

Appendix D provides guidance in this area. Incorporate an effective feedback loop that promotes and ensures continual process improvement. This includes measurable and meaningful indicators and a culture that encourages feedback and capturing lessons learned. Appendix E provides industry standardized indicators to promote comparison of performance among stations, along with other recommended indicators that have proven useful to determine and improve the health of the work management process.

Provide a methodology for a tiered approach to planning and scheduling. This ensures the proper attention is placed on station activities. Appendixes F and G provide guidelines that outline a graded approach to planning and scheduling. Provide a cyclic, repetitive process that incorporates a methodology to manage risk. A typical week scheduling process is illustrated in Appendix B.

The definition of Other maintenance has been expanded to include nondeficiency, proactive station equipment improvements and, as such, is reflective to some degree of active asset management at work. To provide better clarity, a new Appendix H has been added to provide specific examples of how distinctions between Corrective, Elective, and Other maintenance could be made. An SSC should be considered failed or significantly degraded if the deficiency is similar to any of the following examples: This normally includes any deficiency that requires a basis for continued operation as defined in NRC Generic Letterand should be considered as corrective maintenance.

Examples include the following: Examples are as follows: Emergent work is any work that is added to the station master work schedule after schedule freeze. This classification will not include FIN or short-cycle work process work. The Functional Equipment Group FEG is the bundling of multiple equipment subcomponents around a major component with a common system isolation characteristic or out-of-service consideration.

For example, a major pump FEG would consist of the motor or drive unit, the pump assembly, support or auxiliary systems such as oil cooling or seal cooling components, support instrumentation, and power supplies. In that way, all work associated with all the individual subcomponents could be identified and scheduled at the same time.

Some stations apply the concept of FEG to a broader range and include all the equipment that would be bounded by typical system clearance boundaries. Fix-It-Now FIN Teams are special cross-functional work teams assembled as a selfsufficient work group capable of independently performing work without outside support. These teams accomplish work outside the normal schedule on a real-time and immediate basis.

Stations establish work limitation guidance for the FIN teams to ensure that high-risk and complex work activities are subject to the full planning, preparation, and review process. The minor deficiency monitoring MDM process is a process for the management of very low-level maintenance deficiencies whose significance is so low that it would not prudently warrant taking the equipment out of 298 to repair by itself.

Stations shall ensure that MDM items are clearly identified and tracked in such a manner such as functional equipment group FEG or formal tracking and monitoring programs to allow ready 982 and consideration for inclusion if the equipment is removed from service for other reasons, or if inpl degradation would raise the significance of the deficiency.

Stations must ensure that periodic cross-functional reviews are performed minimum annual review on the total inventory of MDM maintenance activities to ensure that the significance of these deficiencies, individually or in the aggregate, has not changed and that they are correctly grouped in 9928 category.

Minor maintenance is a methodology for implementing work that is minor in scope and does not affect nuclear safety functions or increase the ijpo of a plant transient. Normally, work implemented and processed under minor maintenance would not require significant station support for tools and consumables identification, station clearances, and radiological work permits or controls.

Little to no package planning would be required, as the work would be accomplished under what is characterized as skill-of-the-craft.

INPO (AP-928) Conference

Minor maintenance is not a characterization of work such as corrective, elective, or other maintenance and could be included within any of these characterizations. On-line maintenance is maintenance that will be performed with the main generator connected to the grid.

On-Line Material Condition Backlog The total on-line material condition backlog consists of all open corrective and elective maintenance tasks that will be worked with the unit on line. This value should represent the total inventory of degraded conditions on power block station equipment.

Stations should periodically perform cumulative effect analyses or reviews of this listing to ensure that station vulnerabilities are ibpo masked by small issues that compound each other. Other maintenance is ibpo of work not reflecting a material condition deficiency on station power block equipment.

The following would be included in this classification: See Appendix D for an example. Because of the broad and varied nature of the work in this grouping, some stations may choose to subcharacterize this grouping for more detailed assessment or management of the work.

The following tasks are examples of other maintenance: Power block equipment includes all SSCs required for the safe and reliable operation of the station. It will include all safety-related and balance-of-plant system and components required for the operation of the station, including radioactive waste processing and storage and switchyard equipment maintained by the station. Systems, structures, or components required to maintain federal or state regulatory compliance should be included in this grouping.

It is recognized that this distinction may vary among stations. This applies even if the station uses a preplanned preventive maintenance task to correct the deficiency.

Grace period preventive maintenance is any preventive maintenance task that is to be performed beyond pa original due date but prior to the late date for that activity. Normally, this time period due date to late date is an additional 25 percent of the original schedule interval for the PM task.

No engineering evaluation is required. The grace period is provided as reasonable flexibility to allow for alignment with surveillance activities and functional equipment group bundling and to better manage the use of station resources. A deferred preventive maintenance task is a preventive maintenance task that exceeds its original late date with an approved engineering evaluation determining the acceptability for extension to a new due date, prior to the ao late date being exceeded.

A delinquent overdue preventive maintenance task is a preventive maintenance task that exceeds its late date grace period without an approved extension or deferral.

The schedule development process is the multiweek process including work package preparation, walkdown, schedule development, risk assessment, and support coordination for example, parts procurement or engineering resolution prior to the schedule implementation week.

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The short-cycle process is used for the management and implementation of work as defined in Appendix C of this document. Short-cycle work is included in the station integrated schedule. Toolpouch maintenance is a methodology by which work is accomplished that 9288 no initiating work documents. See Appendix A for examples of work activities that can be worked under this process. Work management is the process by which maintenance, modifications, surveillances, testing, engineering support, and any work activities that require plant coordination or schedule integration are implemented.

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Home Etimologias-grecolatinas Etimologias-grecolatinas July 15, Lirbos El presente texto se ha dividido en tres unidades: Teatro romano restaurado en Sagunto, colonia griega aliada de Roma. O como se expresan los marinos: Que tiene intervalos regulares de tiempo.

Etimologias-grecolatinas

Del mismo tiempo, al mismo tiempo. Estudio del tiempo; periodo determinado de tiempo en la historia. Con este sentido interviene en las siguientes palabras: Tratado de las enfermedades. Son verbos que provienen del sustantivo griego: Aunque en la vida diaria se usan en sentido inverso, ya que glosar tiene el sentido de almacenar o guardar un texto sin hacer mayores comentarios; y desglosar es analizar un texto y hacer comentarios sobre el mismo.

La lengua griega es muy brillante. Concha nasalis inferior Cornete inferior linros la nariz. Contesta las siguientes preguntas. Escribe palabras con el prefijo stimologias.

Cada rama del saber, el arte, la industria, las profesiones, etc. Para expresar los conocimientos, las ciencias emplean palabras llamadas: Incluye al vasco y al etrusco. Tamil y lenguas habladas por pueblos de raza negra que habitan el sureste de la India. Se originan de ella: Se habla en Australia. La rama india antigua comprende: La rama irania comprende el antiguo persa, el zendo y el pehlevi. Se forma con dos grupos de lenguas: La eslava oriental abarca el gran ruso, el cual da origen al ruso moderno.

Rama hitita Un pueblo anterior a los fenicios fue el de los hititas quienes habitaron varios siglos antes de Cristo en Asia Menor. Su lengua fue el hitita. Rama griega Compuesta por los idiomas hablados en Grecia.

Entre quienes la utilizaron, se encuentran: Con esta lengua se escribieron los poemas de Alceo y Safo. El osco, hablado en Lucania y Campania sur de Italia. Las regiones donde se habla son: Se habla en Portugal, Angola, Mozambique y Brasil. Se habla en Italia y comprende varios dialectos, entre ellos el napolitano y el veneciano.

Se habla en Rumania, Macedonia y Albania.

Estas lenguas se diversificaron debido a las siguientes causas: Hablado al norte de Francia. Se ubica en algunas regiones de Irlanda y Escocia. Responde las siguientes preguntas. La rama que dio lugar a las lenguas romances es la: A ella pertenece el ruso. Desde la muerte de Augusto a la de Marco Aurelio.

En la Edad Media y en el Renacimiento fue empleado como lengua docta. Con los siguientes colores, marca las zonas donde se ubican las lenguas romances en Europa. Escribe el nombre de las lenguas romances que se ubican en el Continente Americano. Elabora veinte oraciones empleando algunos de los arabismos citados en el vocabulario anterior. Zacazonapan tzompantli, percha de calaveras, zacatl, zacate: En el lugar de agua caliente.

En la cima o meseta de los guajes. Zacatecas zacatl, zacate, tepetl, cerro: En el cerro del zacate. Lugar de las estrellas.

Atzalan atl, agua, zalam, entre: Atotonilco atl,agua, totonqui, caliente, co, en el lugar: Donde hay agua caliente. Chicontepec chicome, siete, tepetl, cerro, co, en el lugar: Lugar de los siete cerros. Nautla nahui, cuatro, tlan, lugar: Donde abundan las mujeres.

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Yecuatla yey, tres, coatl, culebra, tlan, en el lugar: En el lugar de las culebras. Tonayan tonatiuh, amanecer, yan, lugar en: Lugar en donde amanece. Investiga el significado liibros los siguientes americanismos lenguas caribe y haitana. Anota el significado de las siguientes palabras de lenguas sudamericanas. Destacan nombres propios, palabras relacionadas con la vida y costumbres de este pueblo y nombres en torno al trabajo religioso.

Anota veinte nombres propios que provengan del idioma hebreo y expresa sus correspondientes significados. Nombres propios Significados 6 Elementos de lenguas modernas italianismos, galicismos y anglicismos Italianismos A partir del renacimiento, nuestra lengua se ha enriquecido con palabras procedentes del italiano, por ejemplo: Anota diez palabras de origen italiano y especifica su significado Italianismo Significado II. Elabora diez oraciones en las cuales ubiques los italianismos que anteriormente escribiste.

Libfos en definitiva el mayor impulso fue la gran influencia de la literatura francesa. Arabismo A Actividad 7. Elabora un cuadro comparativo en el que presentes diez palabras cultas y diez populares, indicando el significado de cada una.

Palabras cultas Palabras populares Significado II. Contesta las siguientes preguntas: No importa que se repitan letras. Aparecen las Glosas silenses y las Glosas emilianenses. Camacho Becerra, Heriberto et al. Tarsicio y Pimentel Saussure, Ferdinand de, Historia Universal-Roma, Tomo vi. El alfabeto o abecedario latino consta de veinticinco letras se dividen en vocales y consonantes. Las indiferentes llevan superpuestos los dos signos.

Las consonantes se clasifican en: La ll, compuesta de dos eles. La j se pronuncia como y: La t seguida de i y de otra vocal, suena c: Excepto cuando la t va precedida por s, x u otra t: La H no suena: Schola magna hic est? Los estudiantes hacen la tarea. La tarea es hecha por los estudiantes. Con estas palabras primitivas forma una familia de palabras. Parte de la palabra que no cambia y con ella se pueden formar familias de palabras: Etimologiax palabras primitivas son las que:

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